abstract：:The structure of spiroplasmas in the Chinese mitten crab associated with tremor disease was studied by transmission electron microscopy techniques, including both negative staining and ultrasectioning. A spiral structure, which is a typical form of spiroplasmas, could be detected and the observations showed for the fi...

journal_title：Research in microbiology

authors： Wang W,Chen J,Du K,Xu Z

更新日期：2004-10-01 00:00:00

abstract：:Association with a surface in a structure known as biofilm is the prevailing microbial lifestyle. Here we show the kinetics of biofilm formation of Escherichia coli W3110 in static cultures growing under aerobic or anaerobic conditions. Aerobically growing cells in LB medium started to produce detectable amounts of bi...

journal_title：Research in microbiology

authors： Colón-González M,Méndez-Ortiz MM,Membrillo-Hernández J

更新日期：2004-09-01 00:00:00

abstract：:Development of effective strategies for treatment of candidiasis and other fungal diseases has posed a challenge, considering the increase in opportunistic fungal infections in HIV-positive and immunocompromised patients. The in vitro antifungal activity of essential oil from Ocimum gratissimum was investigated in ord...

journal_title：Research in microbiology

authors： Nakamura CV,Ishida K,Faccin LC,Filho BP,Cortez DA,Rozental S,de Souza W,Ueda-Nakamura T

更新日期：2004-09-01 00:00:00

abstract：:Outer membrane vesicle formation occurs during Gram-negative bacterial growth. However, natural production of large amounts of outer membrane vesicles has only been described in a few bacterial genera. The purified vesicles of some bacterial pathogens have shown potential applications in vaccinology and in antibiotic ...

journal_title：Research in microbiology

authors： Henry T,Pommier S,Journet L,Bernadac A,Gorvel JP,Lloubès R

更新日期：2004-07-01 00:00:00

abstract：:Integrative and conjugative elements (ICEs) are self-transmissible mobile genetic elements that are increasingly recognized to contribute to lateral gene flow in prokaryotes. ICEs, like most temperate bacteriophages integrate into the genome and like conjugative plasmids disseminate by conjugative transfer to new host...

journal_title：Research in microbiology

authors： Burrus V,Waldor MK

更新日期：2004-06-01 00:00:00

abstract：:Quorum sensing is a cell-density-dependent regulatory mechanism which, in Gram-negative bacteria, usually involves the production and detection of N-acyl homoserine lactones (HSLs). In the last four years HSL-dependent quorum sensing has been identified in members of the Burkholderia cepacia complex, and this mini-rev...

journal_title：Research in microbiology

authors： Venturi V,Friscina A,Bertani I,Devescovi G,Aguilar C

更新日期：2004-05-01 00:00:00

abstract：:The sequence of a 4.6-kb region of DNA from Corynebacterium glutamicum ATCC 13869 lying upstream from the ftsQ-ftsZ region has been determined. The region contains four genes with high similarity to the murD, ftsW, murG, and murC genes from different microorganisms. The products of these mur genes probably catalyse se...

journal_title：Research in microbiology

authors： Ramos A,Honrubia MP,Vega D,Ayala JA,Bouhss A,Mengin-Lecreulx D,Gil JA

更新日期：2004-04-01 00:00:00

abstract：:The insertion of proteins into membranes generally requires the assistance of membrane proteins. A protein, designated Omp85 in Neisseria meningitidis, was shown to be required for the assembly of bacterial outer-membrane proteins. The protein is essential for the viability of the bacteria and is ubiquitous among Gram...

journal_title：Research in microbiology

authors： Voulhoux R,Tommassen J

更新日期：2004-04-01 00:00:00

abstract：:A gene encoding a sixth chitin synthase (Umchs6, sequence GenBank accession No. ) from the plant pathogenic hemibasidiomycete Ustilago maydis (DC.) Cda. was isolated and characterized. The predicted protein is 1103 amino acids in length with a calculated molecular mass of 123.5 kDa. a2b2 null mutants were obtained by ...

journal_title：Research in microbiology

authors： Garcerá-Teruel A,Xoconostle-Cázares B,Rosas-Quijano R,Ortiz L,León-Ramírez C,Specht CA,Sentandreu R,Ruiz-Herrera J

更新日期：2004-03-01 00:00:00

abstract：:Heterotrophic microaerophilic bacteria, a diverse and ubiquitous group, are specialized for growth in O2 limited environments. Until recently, microaerophilic bacteria have been grouped with obligate aerobic organisms whose metabolic rates slow in response to physiological O2 limitation. In contrast, microaerophilic b...

journal_title：Research in microbiology

authors： Ludwig RA

更新日期：2004-03-01 00:00:00

abstract：:From the nose of a patient with aspiration pneumonia, we isolated a new bacterium by amoebal co-culture using Acanthamoeba polyphaga. Based on phenotypic, 16S rRNA and phylogenetic analysis, we show that this isolate represents a new species within the genus Rhodobacter and and we have named it Rhodobacter massiliensi...

journal_title：Research in microbiology

authors： Greub G,Raoult D

更新日期：2003-11-01 00:00:00

abstract：:Bacteria of the genus Klebsiella are important opportunistic pathogens responsible for nosocomial infections that are increasingly resistant to antimicrobial agents. Distinctive identification of the species K. oxytoca, K. pneumoniae, K. planticola, K. ornithinolytica and K. terrigena is difficult based on phenotypic ...

journal_title：Research in microbiology

authors： Kovtunovych G,Lytvynenko T,Negrutska V,Lar O,Brisse S,Kozyrovska N

更新日期：2003-10-01 00:00:00

abstract：:Rhizobium leguminosarum bv. trifolii synthesizes extracellular polysaccharide (EPS) that is postulated to be a biologically active signalling molecule in clover symbiosis. A group of seven exopolysaccharide-deficient (Exo), non-nitrogen-fixing mutants of R. leguminosarum bv. trifolii strain 24.1 isolated by transposon...

journal_title：Research in microbiology

authors： Janczarek M,Skorupska A

更新日期：2003-07-01 00:00:00

abstract：:A new actinomycete strain designated US24 producing antibacterial activities against Gram-positive and Gram-negative bacteria was isolated from Tunisian soil. Culture characteristic studies strongly suggested that the US24 strain belonged to the genus Streptomyces. Analysis of the nucleotide sequence of the 16S rRNA g...

journal_title：Research in microbiology

authors： Mellouli L,Ben Ameur-Mehdi R,Sioud S,Salem M,Bejar S

更新日期：2003-06-01 00:00:00

abstract：:Recent studies suggest that viruses are the most numerous entities in the biosphere; bacteriophages, the viruses that infect Eubacteria and Archaea, constitute a substantial fraction of this population. In spite of their ubiquity, the vast majority of phages in the environment have never been studied and nothing is kn...

journal_title：Research in microbiology

authors： Desplats C,Krisch HM

更新日期：2003-05-01 00:00:00

abstract：:The Pseudomonas aeruginosa protein AlgR2 (AlgQ) was originally identified as a regulatory protein implicated in alginate production. It also regulates the synthesis of polyphosphate as well as of a variety of secretable virulence factors, upregulating neuraminidase and siderophore synthesis and downregulating rhamnoli...

journal_title：Research in microbiology

authors： Ledgham F,Soscia C,Chakrabarty A,Lazdunski A,Foglino M

更新日期：2003-04-01 00:00:00

abstract：:Multidrug transporters and their transcriptional regulators are key components of bacterial multidrug resistance (MDR). How these multidrug binding proteins can recognize such chemically disparate compounds represents a fascinating question from a structural standpoint and an important question in future drug developm...

journal_title：Research in microbiology

authors： Schumacher MA,Brennan RG

更新日期：2003-03-01 00:00:00

abstract：:The possibility of using kaolinite-immobilized plasmodium fragments of Physarella oblonga (Berk. & Curt.) Morgan to maintain their metabolic activity was examined. The immobilization process was carried out with 1 mg of plasmodium of P. oblonga entrapped in 10 g of kaolinite. Sodium acetate (1 mM) was used as a metabo...

journal_title：Research in microbiology

authors： Ribeiro SM,Cavalcanti LH,Pereira EC,Silva NH

更新日期：2003-01-01 00:00:00

abstract：:Benzene is biodegraded in the absence of oxygen under a variety of terminal electron-accepting conditions. However, the mechanism by which anaerobic benzene degradation occurs is unclear. Phenol and benzoate have been consistently detected as intermediates of anaerobic benzene degradation, suggesting that the hydroxyl...

journal_title：Research in microbiology

authors： Coates JD,Chakraborty R,McInerney MJ

更新日期：2002-12-01 00:00:00

abstract：:Several cDNA fragments corresponding to transcripts differentially expressed under conditions that favor mycelial growth of Candida albicans were identified by the "differential display" technique. One of these was cloned and used as a probe to rescue the full gene from a genomic library of the fungus. The sequence id...

journal_title：Research in microbiology

authors： Orozco I,Ortiz L,Elorza MV,Ruiz-Herrera J,Sentandreu R

更新日期：2002-11-01 00:00:00

abstract：:Bacteriophages must destroy the bacterial cell wall to lyse their host and release their progeny into the environment. There are at least two distinct mechanisms by which phages destroy the cell wall. Bacteriophages with large genomes use a holin-endolysin system, while bacteriophages with small genomes encode a singl...

journal_title：Research in microbiology

authors： Bernhardt TG,Wang IN,Struck DK,Young R

更新日期：2002-10-01 00:00:00

abstract：:The present mini-review summarizes data in selected fields of basic genetics which were exclusively obtained in agreement with the principles of SOS Chromotest methodology and with Escherichia coli PQ37 sfiA::lacZ as a tester strain. ...

journal_title：Research in microbiology

authors： Vasilieva S

更新日期：2002-09-01 00:00:00

abstract：:Azo dye derivatives of azobenzene constitute the largest group of dyes used in the textile industry and possess recalcitrant chemical groups, such as those of azo and sulphonic acid. Some microorganisms are able to degrade these aromatic compounds. In the present work, decolourisation of culture media containing azo d...

journal_title：Research in microbiology

authors： Martins MA,Queiroz MJ,Silvestre AJ,Lima N

更新日期：2002-07-01 00:00:00

abstract：:Paralytic shellfish toxins (PSTs) are sodium channel blocking (SCB) toxins, produced by cyanobacteria, as well as by marine dinoflagellates and their associated bacteria, and cause serious health and economic concern worldwide. In a previous study, approximately 70% of the bacteria enriched from PST-contaminated shell...

journal_title：Research in microbiology

authors： Vásquez M,Grüttner C,Möeller B,Moore ER

更新日期：2002-07-01 00:00:00

abstract：:This study evaluated two generic polymerase chain reaction (PCR) protocols, and nine subtyping protocols and three PCR-restriction fragment length polymorphism (RFLP) protocols for detection of stx genes. The PCR protocols were evaluated by testing 12 reference isolates and 496 field strains of Shiga toxin-producing E...

journal_title：Research in microbiology

authors： Ziebell KA,Read SC,Johnson RP,Gyles CL

更新日期：2002-06-01 00:00:00

abstract：:Type IV secretory systems are transmembrane bacterial multiprotein complexes. They are pivotal for conjugation, bacterial-induced plant tumour formation, toxin secretion and mammalian pathogen intracellular activity. These systems are involved in the spread of antibiotic resistance genes among bacteria by enabling con...

journal_title：Research in microbiology

authors： Gomis-Rüth FX,de la Cruz F,Coll M

更新日期：2002-05-01 00:00:00

abstract：:The removal of water through air drying damages membranes, proteins and nucleic acids and is lethal to the majority of organisms. Nevertheless, some vegetative cells of bacteria and cyanobacteria survive extreme desiccation. Understanding the mechanisms of their desiccation tolerance is an important issue in cell biol...

journal_title：Research in microbiology

authors： Billi D,Potts M

更新日期：2002-01-01 00:00:00

abstract：:The promoter probe mini-Tn5-luxAB-tet was used to create a luxAB transcriptional fusion responding to fluorene in the fluorene utilising bacterium Sphingomonas sp. LB126. The mutant strain, named L-132, was impaired in fluorene utilisation and strongly emitted light upon addition of fluorene to the growth medium. L-13...

journal_title：Research in microbiology

authors： Bastiaens L,Springael D,Dejonghe W,Wattiau P,Verachtert H,Diels L

更新日期：2001-12-01 00:00:00

abstract：:OprI, a small outer membrane lipoprotein from Pseudomonas aeruginosa, can be produced in large amounts and anchored at the surface on Escherichia coli cells. A four-time repeated (His-Cys) motif was fused to the C-terminal part of OprI. After induction, E. coli cells harbouring the recombinant oprI gene became more se...

journal_title：Research in microbiology

authors： Bouia A,Kholti A,Saghi M,Cornelis P

更新日期：2001-11-01 00:00:00

abstract：:The [URE3] element of the yeast Saccharomyces cerevisiae results from the presence of an altered form of the nitrogen regulatory protein Ure2. This altered form acts as an infectious protein (prion). Genes affecting [URE3] initiation and propagation should give valuable information about prion diseases as well as othe...

journal_title：Research in microbiology

authors： Edskes HK

更新日期：2001-09-01 00:00:00

abstract：:The outer membrane of gram-negative bacteria acts as a barrier against harmful lipophilic compounds and larger molecules unable to diffuse freely through the porins. However, outer membrane proteins together with the Tol-Pal and TonB systems have been exploited for the entry of macromolecules such as bacteriocins and ...

journal_title：Research in microbiology

authors： Lloubès R,Cascales E,Walburger A,Bouveret E,Lazdunski C,Bernadac A,Journet L

更新日期：2001-07-01 00:00:00

abstract：:Evidence of increasing resistance to antibiotics in soil and other natural isolates highlights the importance of horizontal transfer of resistance genes in facilitating gene flux in bacteria. Horizontal gene transfer in bacteria is favored by the presence of mobile genetic elements and by the organization of bacterial...

journal_title：Research in microbiology

authors： Nwosu VC

更新日期：2001-06-01 00:00:00

abstract：:ATP binding cassette (ABC) systems constitute one of the most abundant superfamilies of proteins. They are involved not only in the transport of a wide variety of substances, but also in many cellular processes and in their regulation. In this paper, we made a comparative analysis of the properties of ABC systems and ...

journal_title：Research in microbiology

authors： Dassa E,Bouige P

更新日期：2001-04-01 00:00:00

abstract：:In contrast to bacteria, mitochondria contain only a few ATP binding cassette (ABC) transporters in their inner membrane. The known mitochondrial ABC proteins fall into two major classes that, in the yeast Saccharomyces cerevisiae, are represented by the half-transporter Atm1p and the two closely homologous proteins M...

journal_title：Research in microbiology

authors： Lill R,Kispal G

更新日期：2001-04-01 00:00:00

abstract：:The interest of probiotics as remedies for a broad number of gastrointestinal and other infectious diseases has gained wide interest over the last few years, but little is known about their underlying mechanism of action. In this study, the probiotic activities of a human isolate of Lactobacillus casei subsp. rhamnosu...

journal_title：Research in microbiology

authors： Forestier C,De Champs C,Vatoux C,Joly B

更新日期：2001-03-01 00:00:00

abstract：:The increase in genetic variability of a population can be selected during adaptation, as demonstrated by the selection of mutator alleles. The dynamics of this phenomenon, named second-order selection, can result in an improved adaptability of bacteria through regulation of all facets of mutation and recombination pr...

journal_title：Research in microbiology

authors： Tenaillon O,Taddei F,Radmian M,Matic I

更新日期：2001-01-01 00:00:00

abstract：:Several specific primers for the nifH gene were tested with different pure telluric N2-fixing strains. A PolF/PolR primer set provided successful amplification of 19 representative N2-fixing strains. Three restriction enzymes, HaeIII, NdeII and MnlI, chosen for restriction fragment length polymorphism (RFLP) analyses,...

journal_title：Research in microbiology

authors： Poly F,Monrozier LJ,Bally R

更新日期：2001-01-01 00:00:00

abstract：:The trehalose operon of Pseudomonas fluorescens ATCC 17400 consists of treP, treA and treR. The gene treP codes for a putative enzyme II subunit of the phosphotransferase system that catalyzes the phosphorylation of trehalose together with its translocation across the cell membrane and treA encodes a putative phosphot...

journal_title：Research in microbiology

authors： Matthijs S,Koedam N,Cornelis P,De Greve H

更新日期：2000-12-01 00:00:00

abstract：:DNA-DNA hybridization showed that the Brucella strains recently isolated from marine mammals belong to the monospecific genus Brucella (more than 77% DNA relatedness). Ribotyping (HindIII rDNA restriction patterns) showed that they may represent a separate subgroup (marine type) specifically associated with marine mam...

journal_title：Research in microbiology

authors： Verger JM,Grayon M,Cloeckaert A,Lefèvre M,Ageron E,Grimont F

更新日期：2000-11-01 00:00:00

abstract：:Brucella spp. are Gram-negative, facultative intracellular bacteria that cause a zoonotic world-wide disease. As in other Gram-negative bacteria, its S-LPS (smooth lipopolysaccharide) is a major determinant of virulence. The Brucella melitensis 16M LPS O-antigen is a homopolymer of 4-formamido-4,6, dideoxymannose. In ...

journal_title：Research in microbiology

authors： Godfroid F,Cloeckaert A,Taminiau B,Danese I,Tibor A,de Bolle X,Mertens P,Letesson JJ

更新日期：2000-10-01 00:00:00